Study on isolation, characteristic and distribution of pediatric leukemic stem cell using NOG mouse model

来自白血病患者的初级样本成功地移植到小鼠体内，这些植入的白血病细胞能够连续移植到二、三级受体体内。形态学和FACS分析显示，通过连续移植，高达80%的血嵌合体和保守的原始细胞表型。此外，肝、脾、肾等髓外器官显示白血病的侵袭与供体疾病一致。肝组织免疫组织化学分析显示，SDF-1仅在胆管上皮细胞中表达。此外，我们在体内和体外使用CXCR4抑制剂，直接证明了SDF-1/CXCR4轴在我们的模型中的作用。我们对SDF-1/CXCR4轴在肝脏中参与的研究可能会为针对髓外部位的治疗提供新的途径，从而完全克服白血病。

Primary samples from patients with leukemia were successfully engrafted into mice, and those engrafted leukemic cells were able to be serially transplanted into secondary, tertiary recipients. Morphological and FACS analyses revealed as high as >80% blood chimerism and conserved blast phenotypes through serial transplantations. Moreover, extramedullary organs including liver, spleen and kidney showed the leukemic invasion consistent with donor disease. Immunohistological analysis of liver revealed that SDF-1 was detectable only in bile duct epithelial cells. In addition, we demonstrated directly the effect of SDF-1/CXCR4 axis in our model by using the CXCR4 inhibitor both in vivo and in vitro. Our study on the involvement of SDF-1/CXCR4 axis in liver could rink to the novel therapies which target the extramedullary sites in order to perfectly overcome leukemia.