Rapid diagnosis for tiny sentinel node metastasis in malignant melanoma using RT-LAMP method

恶性黑色素瘤一般对化疗和放射治疗耐药，是预后最差的恶性肿瘤之一。本研究的目的是建立恶性黑色素瘤微小前哨淋巴结转移的快速诊断。因此，我们对大约30例黑色素瘤患者进行了前哨淋巴结活检，共50例。82%的病例RT-PCR诊断与病理诊断相符，18%的病例RT-PCR诊断与病理诊断不一致。这一结果进一步证实了RT-PCR诊断恶性黑色素瘤微小前哨淋巴结转移是一种非常有用的方法。此外，我们还考察了RT-LAMP方法的可行性，表明RT-LAMP方法和RT-PCR方法检测恶性黑色素瘤微转移的能力几乎相同。我们认为还需要进一步的实验来改进RT-LAMP方法的条件。

Malignant melanoma is generally resistant for chemotherapy and radiation therapy, and one of malignant tumors with the worst prognosis. The purpose of this study is to establish rapid diagnosis for tiny sentinel node metastasis in malignant melanoma. As a result, we have performed sentinel node biopsies about approximate 30 cases of melanoma patients, resulting in total of 50 cases. In 82% of cases, RT-PCR diagnosis is correspond to pathological diagnosis while RT-PCR is different from pathological diagnosis in 18%. This result further confirms that RT-PCR diagnosis for tiny sentinel node metastasis in malignant melanoma is very useful method. In addition, we examined feasibility of RT-LAMP method and showed that RT-LAMP and RT-PCR methods have almost the same ability to detect tiny metastasis of malignant melanoma. We think we need further experiments to improve conditions of RT-LAMP method.