The new development of ovulation inductor associated with the expression of PTEN in human granulosa cells.

颗粒细胞增殖，然后进行分化;在终末卵泡生长期间观察到这些过程之间的反向关系。但是这种增殖/分化的转变还没有完全理解。我们发现，在人黄体化颗粒细胞中，人绒毛膜促性腺激素可诱导PTEN表达。用hCG预处理减弱IGF-1诱导的AKT磷酸化和细胞增殖。此外，我们评估了胰岛素治疗对PTEN表达的诱导和PTEN对IGF-I诱导的颗粒细胞增殖的影响，以及PCOS和非PCOS患者卵泡液中PTEN水平与胰岛素浓度的相关性。

Granulosa cells proliferate and then undergo differentiation ; an inverse relationship between these processes is observed during terminal follicular growth. But this proliferation/differentiation transition is not fully understood. We show that PTEN was induced with hCG treatment in human luteinized granulosa cells. Pretreatment with hCG attenuated IGF-1-induced phosphorylation of AKT and cell proliferation. Furthermore, we assess the induction of PTEN expression with insulin treatment and effects of PTEN on IGF-I-induced granulosa cell proliferation as well as the correlation of PTEN levels with the concentration of insulin in follicular fluid in PCOS and non-PCOS patients.