Assessment of non-invasive biomarkers for evaluating oocyte quality by proteomic analyses

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Non-invasive biomarkers for evaluating oocyte quality might be helpful to improve success rates of assisted reproduction technology. To identify specific biomarkers of the good quality oocytes and bioactive peptides/proteins possibly involved in folliculogenesis. We collected 24 follicular fluids (FFs) from12 patients who undergone IVF-ET at Nagoya University Hospital. Proteomic analyses were performed by LC/MS. Among all quantitatively-detected proteins, 476proteins were present in both FFs derived from oocytes of resulting in pregnancy and from degenerated oocytes. 18 proteins were present only in FFs derived from oocytes resulting in pregnancy and 9 proteins were present only in FFs derived from degenerated oocytes. The proteomic analysis using FFs revealed that FFs contain many proteins. Analysis of these peptides/proteins might be helpful to identify biomarkers implicated in folliculogenesis and oocyte quality.