Cooperative interactions of a subgroup of the R2R3-MYB transcription factors in controlling phenylpropanoid accumulation

与动物相比，植物含有MYB蛋白亚家族，其特征在于R2 R3型MYB DNA结合结构域。对A. thaliana含有高度保守的明确定义的MYB结构域。该基因家族的成员控制几种植物特异性过程，包括响应胁迫的苯丙素类物质的积累。然而，约80%的R2 R3型MYB因子的功能信息缺失。拟议的项目旨在确定一个子集的R2 R3-MYB转录因子在控制苯丙素积累的合作相互作用。我们将确定R2 R3型MYB因子家族成员的积累和定位模式。合作的相互作用将在植物原生质体中通过系统的共转染试验进行功能研究。此外，通过使用表位标记的MYB因子变体来研究植物中的相互作用。标记的MYB蛋白，当它们已被证明补充各自的敲除表型时，将被用于鉴定预期的和潜在的新的相互作用蛋白。

In contrast to animals, plants contain a MYB protein subfamily which is characterised by the R2R3-type MYB DNA-binding domain. The 126 R2R3-MYB factors of A. thaliana contain a clearly defined MYB domain with a high level of conservation. Members of this gene family control several plant-specific processes including the accumulation of phenlypropanoids in response to stress. However, for about 80 % of the R2R3-type MYB factors functional information is missing. The proposed project aims at defining cooperative interactions of a subset of the R2R3-MYB transcription factors in controlling phenylpropanoid accumulation. We will determine accumulation and localisation patterns for members of the R2R3-type MYB factor family. Cooperative interactions will be functionally investigated by systematic co-transfection assays in plant protoplasts. In addition, in planta interactions will by studied by using epitope-tagged MYB factor variants. The tagged MYB proteins will, when they have been shown to complement the respective knock-out phenotype, be used to identify expected and potentially new interacting proteins.