Console and probe heads 600 MHz NMR spectrometer

申请的主要设备旨在使核磁共振核心设施能够执行具有最高相关性的研究项目。有了这项投资，现代高分辨率NMR光谱学的应用范围将大大扩展，因为两个异核氟（19 F）和磷（31 P）将在600 MHz NMR机器上可用。目前，高灵敏度的19 F NMR实验只能在800 MHz NMR机器（配备CP-QCI探针）上进行。它已被证明，使用600 MHz的场强描绘了一个很好的实验设置，以获取19 F NMR数据，由于19 F拥有的固有特性。此外，目前还不能在核磁共振核心设施的600兆赫核磁共振机上获得31 P核的实验数据。基于NMR光谱方法对核苷酸的广泛研究依赖于31 P核的实验可及性。因此，申请的主要设备极大地扩大了实施范围，使其能够解决DNA/RNA研究中追求的研究目标。康斯坦茨大学化学或生物系附属的研究小组积极参与促进跨学科的合作努力。在这里，一个适当的最新装备的NMR基础设施有力地支持了依赖于分子甚至原子尺度上的实验见解的研究项目。例如，可以列出旨在确定细胞环境中蛋白质热力学稳定性和功能的基于氟的研究、蛋白质翻译后修饰的项目以及计算机模拟与核磁共振光谱实验数据相结合的补充研究。对所申请的主要设备进行投资的另一个动机涉及核磁共振核心设施提供的仪器的可用性。目前，有两个低温平台运行（600 MHz配备CP-TCI低温探头（2008年安装）和800 MHz配备CP-QCI低温探头（2009年安装））。使用最先进的室温探头增加了鲁棒性、可靠性，并且显著地增加了可用于600 MHz NMR光谱仪实验工作的温度范围。现有磁场强度为14.1 T的超导磁体（2008年安装）将被保留并进一步使用。申请的主要设备将补偿由于过去15年中探头和控制台技术的技术进步和方法进步而从低温平台到室温设置的过渡。与此同时，NMR活性核的实验可用性将显著扩大（目前：1H，2 H，13 C，15 N;然后：1H，2 H，13 C，15 N，19 F和31 P）。

The major equipment applied for aims to enable the execution of research projects at the NMR core facility that possess highest relevance. With this investment the application spectrum modern high-resolution NMR spectroscopy offers will be significantly expanded as the two heteronuclei fluorine (19F) and phosphor (31P) will be then available at a 600 MHz NMR machine. Currently, highly sensitive 19F NMR experiments can be exclusively conducted on an 800 MHz NMR machine (equipped with CP-QCI probe). It has been shown that using a field strength of 600 MHz depicts an excellent experimental setup to acquire 19F NMR data due to the intrinsic characteristics 19F possesses. Besides, experimental data on 31P nuclei cannot be acquired on the 600 MHz NMR machine at the NMR core facility at the moment. Extensive studies on nucleotides that are based on NMR spectroscopic approaches rely on the experimental accessibility of 31P nuclei. Consequently, the major equipment applied for crucially expands the implementation range enabling to tackle research objectives pursued in DNA/RNA research. Research groups affiliated with the Department of Chemistry or Biology at the University of Konstanz are strongly involved in collaborative efforts promoting interdisciplinarity. Here, an appropriate up to date equipped NMR infrastructure strongly supports research projects that rely on experimental insights at the molecular and even atomic scale. Exemplarily, fluorine-based studies aiming to determine thermodynamic stability and function of proteins in a cellular context, projects on posttranslational modifications of proteins and complementary studies combining computer simulations with NMR spectroscopic experimental data can be listed. Another motivation for the investment in the major equipment applied for concerns the availability of the instruments provided by the NMR core facility. Currently, two cryo platforms are operated (600 MHz equipped with CP-TCI cryo probe (installation in 2008) as 800 MHz equipped with CP-QCI cryo probe (installation in 2009)). Using state-of-the-art room temperature probe heads increases the robustness, dependability and, significantly, the temperature range that can be used for experimental efforts at the 600 MHz NMR spectrometer. The existing superconducting magnet possessing magnetic field strength of 14.1 T (installation in 2008) will be kept and further used. The major equipment applied for will compensate the transition from a cryo platform to a room temperature setup due to technical advancement and methodological progress of probe heads and console technology noted during the past 15 years. In parallel, the experimental availability of NMR active nuclei will be significantly expanded (currently: 1H, 2H, 13C, 15N; then: 1H, 2H, 13C, 15N, 19F und 31P).