Molecular behavior in non-equilibrium and asymmetric domains formed in supported planar lipid bilayers

在亚微米区域原位观察到了分子在支撑平面类脂双层中的扩散行为，并利用固体衬底上的纳米结构对其进行了控制。构建了从毫秒/100 nm到秒/μm的时空顺序的单分子跟踪荧光显微镜，实现了对硅、二氧化钛等不透明衬底和高折射率衬底的单分子跟踪。对2000fps~33fps的脂质扩散的原位观察表明，衬底上的表面纳米结构诱导了所支撑的脂质双层的异常扩散。

Molecular diffusion behavior in supported planar lipid bilayers at submicron regions were observed in situ, and controlled using nanostructures on solid substrates. I constructed a fluorescence microscope for single molecule tracking at the spatiotemporal order over millisecond / 100 nm to second/μm, and achieved the single molecule tracking on opaque substrates and high-refractive substrates such as silicon and titanium dioxide. In situ observation of lipid diffusion at 2000 fps to 33 fps revealed that surface nanostructures on the substrates induced anomalous diffusion in the supported lipid bilayer.