Control of membrane fouling in MBRs on the basis of proteome analysis and measurements of atomic force

本研究对膜生物反应器(MBR)中膜污染相关的蛋白质结构进行了研究。从膜生物反应器处理城市污水的污染膜中提取了引起膜污染的蛋白质。通过对样品前处理方法的改进，实现了双向凝胶电泳法的应用，可以根据分子量和等电点来分离引起膜污染的蛋白质。对分离出的蛋白质进行氨基酸序列分析。结果表明，假单胞菌属细菌的外膜蛋白在膜生物反应器的膜污染演化过程中起着重要作用。

In this study, structure of proteins that were involved in membrane fouling in membrane bioreactors(MBRs) was investigated. Proteins causing membrane fouling were obtained from fouled membranes used in an MBR treating municipal wastewater. Modification of sample pre-treatment enabled application of two-dimensional gel electrophoresis and the protein causing membrane fouling could be separated on the basis of molecular weights and isoelectric points. The separated proteins were subjected to amino acid sequence analysis. It was shown that outer membrane proteins of bacteria belonging to the genus Pseudomonas played an important role in the evolution of membrane fouling in MBRs.