Developmental analysis of the neural activity in the left and right hemispheres using zebrafish

为了研究斑马鱼左右脑半球神经连通性及其活动的差异，我们以斑马鱼左右脑半球的缰状神经回路为研究对象。通过这些分析，我们能够1)通过钙显像观察到左右束细胞活性的差异，2)通过形态学和基因表达分析鉴定出哺乳动物内侧和外侧束的鱼类同源性，3)鉴定出接受背束不对称投射的束间核的输出途径。

We focused on the zebrafish habenular circuit showing the left-right asymmetry in the projection pattern to study the difference in neural connectivity and its activity between left and right hemispheres. Through the analyses, we were able to 1) visualize the difference in the activity of the cells in the left and right habenula with calcium imaging, 2) identify the fish homologue of mammalian medial and lateral habenulae by the hodological and gene expression analyses and 3) identify the output pathway of the interpeduncular nucleus which receives asymmetric projection from the dorsal habenula.