Development of Quantitative Bisulfite Sequencing

胞嘧啶的DNA甲基化被认为是癌症的信号之一。甲基胞嘧啶的检测和定量有可能导致用于预测癌症的遗传诊断技术。亚硫酸氢盐测序法是一种非常著名的甲基胞嘧啶检测方法，但由于DNA在较高的酸度和温度下与高浓度的亚硫酸氢盐反应，存在最终甲基化量的问题。为了开发定量，在较低酸度和较低温度下用低浓度的试剂对DNA进行较温和的反应。

DNA methylation of cytosine is known to be one of the signals for cancer. The detection and quantification of methylcytosines have the possibility of leading to genetic diagnosis technology for prediction of cancer. Bisulfite sequencing is very famous method for detecting methylcytosines, which has problem in quantity of final methylation because of the reaction of DNA with high concentration of bisulfite at higher acidity and higher temperature. To develop the quantification, milder reaction was performed on DNA with low concentrations of reagents at lower acidity and lower temperature.