Visualization analysis of neurotransmission and exocytosis by using novel fluorescent protein and laser optics technology

我们开发了用于神经传递和胞吐作用的新的可视化分析技术，并构建了“体内”光学成像方法，以促进分子基础的阐明。我们使用一种新的荧光蛋白天狼星来产生荧光标记的SNARE蛋白VAMP-Sirius，以便在破骨细胞模型中可视化酸性小泡的动态。一种新的FRET钙离子传感器，Cameleon-Nano Protein，可视化胰腺腺泡细胞钙依赖性胞吐，以及小脑神经元的钙离子动力学。此外，我们成功地在活体小鼠大脑中观察到了更深层次的海马神经元。

We have developed novel visualization analysis techniques for neurotransmission and exocytosis, and constructed “in vivo” optical imaging methods to advance for elucidation of the molecular basis. We generated fluorescently tagged SNARE proteins, VAMP-Sirius by using a novel fluorescent protein Sirius in order to visualize dynamics of acid vesicles in an osteoclast model. A novel FRET Ca^<2+> sensor, Cameleon-nano protein, visualized Ca^<2+>-dependent exocyosis in pancreatic acinar cells, as well as Ca^<2+> dynamics in neurons in cerebellum. Furthermore, we successfully observed hippocampal neurons in deeper layers in live mouse brains.