Design of Scaffold Materials Bearing Stabilization of Gwoth Factors Toward Angiogenesis

在本项目中，聚甘油树状大分子（PGDs）作为蛋白质稳定剂的候选，旨在创造具有生长因子稳定的新型聚合物支架。作为模型蛋白的乙醇脱氢酶（ADH）的热变性在PGDs的存在下被阻止，这表明PGDs起着蛋白质稳定剂的作用。这种影响是由于水结构的调制，这是由核磁共振研究澄清。此外，等温滴定量热法研究了PGDs与碱性氨基酸的相互作用，并发现PGDs能明显保持碱性成纤维细胞生长因子（bFGF）的生物活性。因此，PGDs作为蛋白质稳定剂是可行的，并且可以应用于承载生长因子稳定的聚合物支架。

In this project, polyglycerol dendrimers (PGDs) were focused as a candidate of protein stabilizers aiming at creation of new polymeric scaffolds bearing growth factor stabilization. Thermal denatulation of alcohol dehydrogenase (ADH), as a model protein, was found to be prevented in the presence of PGDs, suggesting that the PGDs act as a stabilizer of proteins. This effect was due to modulation of water structure, which was clarified by NMR studies. In addition, PGDs could interact with basic amino acids, which was investigated by isothermal titration calorimetric studies, and biological activity of basic fibroblast growth factor (bFGF) was apparently preserved by the addition of PGDs. Therefore, PGDs are feasible to use as a protein stabilizer, and can be applicable as a polymeric scaffold bearing growth factor stabilization.