Regulatory mechanisms of translesion DNA synthesis

人DNA聚合酶eta（Polη）是一种易患癌症的遗传性疾病着色性干皮病变异体的负责基因产物，催化准确和有效的跨损伤DNA合成，并防止由DNA损伤诱导的细胞死亡和癌变。该项目揭示了人Polη具有独特的结构以催化准确和有效的跨损伤DNA合成。该项目还确定了一种新的PCNA泛素化机制，这对跨病变DNA合成的调控及相关机制至关重要。

Human DNA polymerase eta (Polη) is the responsible gene product of a cancer prone genetic disorder, xeroderma pigmentosum variant, catalyzes accurate and efficient translesion DNA synthesis, and prevents cell death and carcinogenesis induced by DNA damages. This project revealed that human Polηhas a unique structure to catalyze the accurate and efficient translesion DNA synthesis. This project also identified a novel mechanism of PCNA ubiquitylation which is crucial for regulation of translesion DNA synthesis and related mechanisms.