Analysis of RACK function in specific nascent peptide-dependent translation arrest

活化的C激酶受体(RACK1)被认为是新生肽依赖的翻译抑制所必需的因子，而RACK1与40S亚基的结合是多碱性氨基酸序列抑制翻译的关键。两个E3泛素连接酶，Ltn1和Not4，参与了蛋白酶体蛋白降解与翻译抑制的耦合，而RACK1需要翻译抑制才能降解Ltn1-去肽网蛋白。

The receptor for activated C kinase (RACK1) was identified as a factor required for nascent peptide-dependent translation arrest, and the binding of RACK1 to 40S subunit is crucial for translation repression by poly-basic amino acid sequence. Two E3 ubiquitin ligases, Ltn1 and Not4, are involved in proteasomal protein degradation coupled to translation arrest, and translation arrest by RACK1 is required for Ltn1-depepdnet protein degradation.