Transcriptional mechanism of noncoding RNA

我们已经证实，启动子衍生的非编码RNA(PncRNA)是从细胞周期蛋白D1基因的启动子区域转录而来的，并抑制该基因的表达(自然434：126,2008)。我们还确定了pncRNA的转录起始点，并纯化了诱导pncRNA转录的转录因子。纯化后获得了5个DNA修复相关蛋白和2个副斑点相关蛋白。这些数据表明，pncRNA的转录需要DNA修复系统，并且转录位于细胞核的副斑点区。这将有助于阐明ncRNA转录的新机制。

We have identified that promoter-derived noncoding RNA (pncRNA) is transcribed from the promoter region of the cyclin D1 gene and represses the expression of the gene (Nature 434: 126, 2008). We have also determined a transcriptional initiation site of pncRNA and purified transcription factors inducing the transcription of pncRNA. The purification provided five proteins involving DNA repair and two of paraspeckle-related proteins. These data suggest that the transcription of the pncRNA requires the DNA repair system and the transcription is located in the paraspeckle area of nuclei. This will enhance elucidation of novel mechanisms of the ncRNA transcription.