Induction of hematopoietic stemcells from iPS cells and the molecular basis in the induction

Geminin被认为调节造血干细胞（HSCs）自我更新或分化产生成熟血细胞的细胞命运决定。然后，我们产生了Geminin-EYFP敲入小鼠，以可视化来自鼠敲入成纤维细胞的Geminin和iPS细胞。我们还通过使用shRNA构建了过表达或敲低Geminin表达的载体系统。我们在此表明，Geminin诱导造血干细胞的静止和未分化状态，但Geminin在后代亚群中下调以诱导分化和增殖，导致大量成熟血细胞的产生。我们进一步分析Geminin在自我更新造血干细胞中的表达谱和作用，旨在产生一种从iPS细胞诱导HSC的新技术。

Geminin is presumed to regulate cell fate decision whether the hematopoietic stem cells (HSCs) self-renew or differentiate to produce mature blood cells. We then generated Geminin-EYFP knockin mice to visualize Geminin and iPS cells from the murine knockin fibroblasts. And we also generated vector systems to overexpress or knock-down Geminin expression by using the shRNA. We here showed that Geminin induces quiescence and undifferentiated states in hematopoietic stem cells but that Geminin is down-regulated in the progeny subpopulations to induce differentiation and proliferation, resulting in production of large amount of mature blood cells. We further analyze expression profile and a role for Geminin in self-renewing hematopoietic stem cells, and aim to generate a new technology for inducing HSCs from iPS cells.