Neuranagenesis factor and comprehensive gene analysis for spinal cord injury

本研究应用Flexercell应变仪和DNA微阵列技术，分离培养15 d龄Sprague-Dawley大鼠胚胎脊髓细胞，研究周期性张应力对脊髓细胞的影响。周期性张应力的应用降低了培养的脊髓细胞的活力显着的剂量和时间依赖性的方式。增加应变或应变率独立与脊髓细胞存活率显着下降。没有明显的证据表明应变水平与应变速率的加性效应。GO分析确定了44个与“细胞凋亡”显著相关的候选基因和17个与“刺激反应”相关的候选基因。KEGG分析确定了丝裂原活化蛋白激酶（MAPK）信号通路的12个基因的表达水平的变化，这些基因通过RT-PCR分析被证实上调。这些数据可能被证明是有用的，因为准确了解神经元基因表达对周期性张应力的反应将有助于开发脊髓损伤的分子治疗方法。

The present study was designed to investigate the effect of cyclic tensile stresses on cultured spinal cord cells using the Flexercell Strain Unit and DNA microarray technology.Spinal cord cells were isolated for culture from 15-day Sprague-Dawley rat embryos. The application of cyclic tensile stress reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. Increasing either the strain or the strain rate independently was associated with significant decreases in spinal cord cell survival. There was no clear evidence of additive effects of strain level with strain rate. GO analysis identified 44 candidate genes which were significantly related to “apoptosis” and 17 genes related to “response to stimulus”. KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated by RT-PCR analysis. These data may prove useful, as the accurate knowledge of neuronal gene expression in response to cyclic tensile stress will help in the development of molecular-based therapies for spinal cord injury.