Stem cells from human-exfoliated deciduous teeth can regenerate central nervous system

我们研究了成人智齿牙髓干细胞(DPSC)和脱落型乳牙干细胞(SHOLD)的多向分化潜能、表型标记表达和转录组特征。结果表明，表达多种神经链标志的SHEAD和DPSC均能分化为神经元、星形胶质细胞、少突胶质细胞、成骨细胞、脂肪细胞和软骨细胞。此外，我们优化了方案，允许SHEAD和DPSC特异性分化为表达多巴胺能神经元的酪氨酸羟基酶。在临床前分析中，我们发现SHOPE和DPSC在治疗脊髓损伤、缺氧性脑损伤和帕金森病方面有显著的疗效。除具有明显的神经再生活性外，植入后8周未观察到移植棚的恶变。可以从剥离的乳牙和阻生的成人智齿中获得棚屋和DPSCs，而不会对健康造成不利影响。因此，它们的临床应用几乎没有伦理上的顾虑。我们认为，牙源性干细胞可能是治疗各种中枢神经系统疾病的一种良好而实用的细胞资源。

We have characterized the multi-differentiation potency, phenotypic marker expression and trascriptome profile of dentalpulp derived stem cell from adult wisdom teeth (DPSC) and exfoliated deciduous teeth (SHED). The data showed that both SHED and DPSC expressing multiple neural linage markers are able to differentiate neuron, astrocyte, oligodendrocyte, osteoblast, adipocyte and chondrocytes. Furthermore, we have optimized the protocol allowing a specific differentiation of SHED and DPSC to tyrosine hydroxylase expressing dopaminerigic neurons. In pre-clinical analysis, we have found remarkable therapeutic benefits of SHED and DPSC for treatment of spinal cord injury, hypoxic brain injury and Parkinson’s disease. In addition to their remarkable neuro-regenerative activities, we did not observe the malignant transformation of engrafted SHEDs 8 weeks after theirimplantation. SHEDs and DPSCs can be obtained from exfoliated deciduous and impacted adult wisdom teeth without adverse health effects. Thus, there are few ethical concerns regarding their clinical use. We propose that tooth-derived stem cells may be an excellent and practical cellular resource for the treatment of various CNS diseases.