Therapeutic strategy for oral cancer based on hypoxia PET imaging

遗传乏氧显像的建立对口腔癌的治疗非常关键。用正电子发射断层扫描(PET)和单光子发射计算机断层扫描(SPECT)观察人钠碘同向转运体(HNIS)基因的表达。本课题组已经建立了稳定表达hNIS的细胞系。我们建立了稳定表达hNIS的癌细胞株(HCT116-hNIS株B)，并成功地用99mTcO4SPECT对小鼠异种移植模型进行了显像。在未来，该模型将提供一种工具来追踪小鼠体内的肿瘤转移，从而阐明转移级联的分子机制。

Establishment of genetic hypoxia imaging is very critical for the treatment of oral cancer. The expression of the human sodium-iodide symporter (hNIS) gene is visualized by positron emission tomography (PET) and single photon emission computed tomography (SPECT). Our group has already established cell lines stably expressing hNIS. We established the cancer cell line stably expressing hNIS (HCT116-hNIS line B), and succeeded in imaging the mouse xenograft model by 99mTcO4SPECT. In the future, this model will provide a tool to track tumor metastasis in vivo in mice so that the molecular mechanism involved in metastatic cascade will be elucidated.