Functional analysis of nectin and afadin system in synaptogenesis

在神经元发育的早期，树突及其分支动态地伸出许多精细的丝状足，逐渐形成复杂的结构，但这些树突丝状足在树突乔木中的作用尚不清楚。MAGUK家族成员ZO-1集中在丝状足-丝状足接触部位。ZO-1的过表达或敲低会影响树突丝状突起的动力学及其相互作用，导致树突形态异常。这些结果表明，ZO-1在培养神经元中调节树突丝状排列动力学，从而调节树突形态发生。

Neuronal dendrites and their branches dynamically protrude many fine filopodia in the early stages of neuronal development thatgradually establish complex structures, but the role of these dendritic filopodia in dendritic arborization is unknown. The MAGUK family member, ZO-1, was concentrated at the filopodia-filopodia contact sites. Overexpression or knockdown of ZO-1 affected thedynamics of dendritic filopodial protrusions and their interactions, causing abnormaldendrite morphologies. These results indicate that ZO-1 regulates dendritic filopodialdynamics, and therefore dendrite morphogenesis, in cultured neurons.