Subtypes ofmicroglia/macrophages relevant to the diagnosis and treatment of brain tumors

胶质瘤浸润性小胶质细胞/巨噬细胞被称为肿瘤相关巨噬细胞（tam）。v- erbb是表皮生长因子受体的一种病毒形式，在S100-β启动子的转录调控下，转基因（TG）大鼠可发生脑肿瘤。我们对人胶质瘤和实验肿瘤中的小胶质细胞/巨噬细胞进行了免疫组织化学和形态计量学分析。基于结果，我们发现在胶质瘤组织中一致发现iba1阳性，具有活化/吞噬细胞形态的TAM。在实质内间变性少突胶质细胞瘤中，肿瘤核心的iba1阳性tam明显比非肿瘤性脑组织的iba1阳性小胶质细胞活化。与人类胶质瘤相比，实验胶质瘤中的大多数tam对CD68、CD163或CD204没有或很少表达，尽管在坏死和增殖血管壁中观察到CD204阳性的tam。综上所述，S-100β-v-erbB TG大鼠可以作为一种有用的动物模型，进一步分析tam在肿瘤细胞增殖、微血管增殖和吞噬方面的作用，并作为治疗恶性胶质瘤的工具，尽管应该注意的是tam向M2表型的极化尚不清楚。

Glioma-infiltrating microglia/macrophages are referred to as tumor-associated macrophages (TAMs). Transgenic (TG) rats expressingv-erbB, which is a viral form of the epidermal growth factor receptor, under transcriptional regulation by the S100-βpromoter develop brain tumors. We carried out immunohistochemical and morphometrical analyses of microglia/macrophages both in human gliomas and in the experimental tumors. Based onthe results, we found that the Iba1-positive, TAM with the morphology of activated/phagocytic cells were consistently found within glioma tissues. Iba1-positive TAMs of tumor core were significantly more activated than Iba1-positive microglia of non-neoplastic brain tissue in intraparenchymal, anaplastic oligodendrogliomas. In contrast to human gliomas, most TAMs in the experimental gliomas showed no or little expression against CD68, CD163, or CD204, although CD204-positive TAMs were observed in necrosis as well as in proliferating vascular wall. In conclusion, S-100β-v-erbB TG rats may serve as a useful animal model for further analysis of TAMs in terms of tumor cell proliferation, microvascular proliferation and phagocytosis, and as a tool for therapeutic use in malignant gliomas, although it should be noted that the polarization of TAMs towards the M2 phenotype remains unclear.