Development of rapid biomarker measurement system for functional analysis of human skin

我一直致力于开发一种非侵入性/非破坏性的人体皮肤瞬时测量系统(皮肤功能分析)，作为皮肤表面出现的包括脂肪酸、糖化最终产物和许多生物分子在内的生理标志物的可测量位置。因此，我主要得到了三个重要的结果：(1)通过在手臂皮肤表面负载一定量的乳酸镁(mg-lac)，皮肤表面组胺的合成量显著减少。持续出现在皮肤表面的MG-Lac似乎具有控制皮肤中组胺合成的作用。(2)1~2小时后，FTIR可以观察到膳食脂肪酸DHA(二十二碳六烯酸)出现在唇部表面。DHA在唇部表面的形态分布存在性别差异，且男性明显高于女性。(3)用傅立叶变换红外光谱仪直接测定人嘴唇朱砂表面糖化终末产物(如羧甲基精氨酸和磷脂酰乙醇胺)，可预测糖尿病患者的糖化血红蛋白。

I have investigated to develop a non-invasive/non-destructive and instantaneous measurement system of human skin (Skin Function Analysis) as a measurable site of physiological marker materials including fatty acids, glycated end-products and many biomolecules appeared on the skin surface. As a consequence, I have obtained mainly three important results:(1) By loading certain amount of magnesium lactate (Mg-lac) on arm skin surface, the amount of skin surface histamine synthesized was significantly decreased. Mg-lac which appears continuously on the skin surface seems to function to control the histamine synthesis in the skin.(2) Dietary fatty acid, DHA (docosahexaenoic acid), could be observed by FTIR to appear on the lip surface after 1~2 hours later. The appearance profile of DHA on the lip surface was different between males and females, and the significant appearance was observed more clearly for males than females(3) The prediction of HbA1c values for diabetes patients became possible by measuring with FTIR directly the glycated end-products (such as carboxymethylated (CM) arginine and phosphatidyl- ethanolamine (PE)) on the human lip vermillion surface, using PLSregression method.