Development of fast and multi-functional biological analysis systembased on the ultra-micro droplet reaction place

利用喷墨技术成功研制了微滴反应场。对喷墨产生液滴的方法进行了广泛的分析，最终找到了最佳的工艺条件。利用液滴反应系统，实现了微全分析系统最重要的特性--尺寸效应。利用该液滴反应系统，实现了生物样品的酶联免疫溶剂法检测系统。研制了入射光、荧光和化学发光的点样测量系统。在此基础上，成功地制备了具有微米和纳米微珠的三维有序结构，并将其用于新型分析工具。采用三维有序微球结构的酶联免疫吸附试验显示，以1/1000试剂消耗量作为反应场所，检测灵敏度大幅提高。最后，我们成功地制备了具有纳米微珠的光子晶体，展示了人眼感知材料的可能性。

Micro-droplet reaction place was successfully developed by using ink-jet. Generation method of droplet from ink-jet was extensively analyzed and finally we have found the optimal conditions. By using droplet reaction system, size effect, which is the most important characteristics of micro-total analysis system, was realized. Using this droplet reaction system, measuring system for bio-samples using enzyme linked immune solvent assay was realized. The system for sample dispensing and measuring system for incident light fluorescent and chemiluminescence was developed. Then, three dimensional ordered structure with micro and nano beads was successfully developed and those structure were used for the novel analytical tool. ELISA using three dimensional ordered micro beads structure showed drastic increase in the detection sensitivity with 1/1000 reagent consumption when it was used as a reaction place. Finally, we have successfully fabricated photonic crystals with nano-bead and showed the possibility of material sensing by eyes.