Integration of pleiotropic signals involved in the telomere integrity

酿酒酵母发芽细胞的CST(Cdc13-Stn1-Ten1)复合体与端粒DNA末端结合，参与端粒复制和保护。在细胞周期的特定时间段，Stn1被CDK磷酸化，并且该磷酸化负调控端粒的伸长。此外，我们还发现，保护功能缺陷的stn1突变体通过重组和端粒酶依赖的机制导致端粒延长。此外，我们发现端粒缩短介导的细胞衰老通过调节TOR信号通路和端粒结合蛋白Rap1来诱导自噬。

CST (Cdc13-Stn1-Ten1) complex of the budding yeast Saccharomyces cerevisiae has been shown to associate with the telomeric DNA ends and to be involved in telomere replication and protection. Stn1 was phosphorylated by Cdk during specific times during the cell cycle, and the phosphorylation regulated telomere elongation negatively. In addition, we found that an stn1 mutant defective in its protective function resulted in telomere elongation via the recombination- and telomerase-dependent mechanisms. Moreover, we found that telomere shortening-mediated cellular senescence induced autophagy by regulating the TOR-signaling pathway and the telomere-binding protein Rap1.