functional evolution of nebulin superfamily in vertebrates

星云蛋白超家族蛋白如LASP、LASP-1、LASP-2、NeBulin、NeBulette和N-RAP具有相似的结构域结构(NeBulin重复序列、LIM结构域和SH3结构域)，被认为是由相同的祖先基因产生的。本研究试图揭示它们在脊椎动物神经和肌肉组织中的功能进化。我们发现LASP-1和LASP-2在斑马鱼中的组织分布不同。在鸡和大鼠的原代神经元中，Lasp-1和Lasp-2的定位不同，而Lasp-2的siRNA和Lasp-2的显性负结构导致了原代神经元丝状足的形态异常。LASP-2在鸡和大鼠神经胶质细胞的囊泡转运、细胞-底物附着和外周星形胶质细胞过程中发挥作用。

Nebulin superfamily proteins such as lasp, lasp-1, lasp-2, nebulin, nebulette, and N-RAP have similar domain structures (nebulin repeats, LIM domain and SH3 domain) and are thought to be generated from the same ancestral gene. This study tried to reveal their functional evolution in nerve and muscle tissues in vertebrates. We found tissue distribution of lasp-1 and lasp-2 were different in zebra fish. Localization of lasp-1 and lasp-2 were different in primary neurons of chicken and rat and siRNA of lasp-2 and dominant negative constructs of lasp-2 resulted morphological abnormality in filopodia in the primary neurons. Lasp-2 play roles in vesicle transport, cell-substrate attachment and peripheral astrocyte process in glial cells of chicken and rat.