Molecular cell biological studies on the evolution of male and female sex in algae.

利用电子显微镜研究了绿藻纲绿色藻类螺旋毛藻、狭腔膜和绿藻纲绿色胸藻的配子融合位点或交配结构的空间排列。在所有物种中，配子的细胞融合位点在一种性别类型中位于眼斑的同一侧，而在相反交配类型中位于眼斑的另一侧。这些结果支持了细胞融合位点或交配结构的性别特异性排列可能是绿藻普遍特征的观点。利用G。胸肌在MID+配子中，交配结构存在于与眼点相同的一侧，而在MID-配子中，交配结构存在于眼点的相对侧。这些结果与莱茵衣藻的结果一致，并表明这些藻类的交配结构的性别特异性排列与MID的存在或不存在密切相关。

Spatial arrangement of gamete fusion site or mating structure was studied in ulvophycean green algae, Chaetomorpha spiralis, Monostroma angicava and chlorophycean green alga Gonium pectoral using electron microsco py. In all species, cell fusion site of the gamete was located on the same side of the eyespot in one sex type while it was located on the other side of the eyespot in the opposite mating type. These results support the proposal that sex specific arrangement of cell fusion site or mating structure may be a universal feature in chlorophyte algae. The relationship between the sex determination gene MID and sex specific arrangement of mating structure was studied using G. pectorale. In the MID+ gametes, mating structure was present on the same side as the eyespot while in the MID- gametes mating structure was present on the opposite side of the eyespot. These results are consistent with that of Chlamydmonas reinhardtii and suggest that the close relationship between presence or absence of MID and sex specific arrangement of mating structure in these algae.