Coordination mechanism of multiple motor proteins : a DNA-protein hybrid nanosystem study

马达蛋白激酶利用ATP产生的能量驱动细胞内的囊泡运输。然而，多个运动蛋白如何相互协调仍然是一个谜。在这里，我们创建了一个混合纳米机器（DNA-Tile-kinesin），使用DNA-Tile作为骨架结构，kinesin作为功能模块。dna - tile -激酶的单分子成像使我们能够评估参与运输的分子数量和多个激酶之间距离的纳米变化对运动性的影响。我们的研究结果表明，参与运输的运动蛋白的数量对跑长有影响，但对速度没有影响。这些结果表明，我们的方法对理解运动蛋白的协调机制的力量。

The motor protein kinesin drive vesicle transport in the cell using energy derived from ATP. However, how multiple kinesin are coordinated each other remains elusive. Here we created a hybrid nanomachine(DNA-Tile-kinesin) using DNA-Tile as the skeletal structure and kinesin as the functional module. Single molecule imaging of DNA-Tile-kinesin hybrid allowed us to evaluate the effects of the number of molecules involved in the transport and nanometer changes in the distance between multiple kinesins on motility. Our results show that number of kinesin participating in the transport affect on run length but not on velocity. These results indicate the power of our approach toward the understanding of coordination mechanism of motor proteins.