The development of the new RNAi vector system that is effective in analysis and therapy of leukemia and cranial nerve disease.

本研究利用T7 RNA聚合酶构建了在细胞质中表达siRNA的RNAi载体系统。pCMV-T7 RNAP和pT 7-RNAi-Lu共转染HeLa细胞和A549细胞后，荧光素酶的表达明显受到pGL 3-control质粒的抑制。pCMV-T7 RNAP和pT 7-RNAi-p53共转染可显著抑制HeLa细胞中p53蛋白的表达。因此，我们能够确认使用T7 RNA聚合酶的新载体系统具有抑制靶基因表达的功能。

In this study, we developed the RNAi vector system which expressed siRNA in cytoplasm using T7 RNA polymerase. The co-transfection of pCMV-T7RNAP and pT7-RNAi-Lu significantly suppressed luciferase expression by pGL3-control plasmid in HeLa cells and A549 cells. Moreover, the co-transfection of pCMV-T7RNAP and pT7-RNAi-p53 significantly suppressed p53 protein expression in HeLa cells. Thus, we were able to confirm that the new vector system using T7 RNA polymerase has function to suppress the expression of target gene.