The source of acetylcholine acting to the renal tubules.

为了阐明对肾脏起作用的乙酰胆碱（ACh）的来源，采用神经示踪法和组织化学方法分别检测了大鼠肾脏的副交感神经支配和胆碱乙酰转移酶（ChAT）的非神经元表达。用微胶囊处理肾神经末梢的逆行神经元示踪剂氟金，延髓和脊髓的副交感神经核均未见阳性细胞。另一方面，乙酰胆碱合成酶ChAT定位于肾皮质集管，在小管中分布不均匀。这些结果表明，大鼠肾脏中非神经元乙酰胆碱可能比神经元乙酰胆碱占优势。

To clarify the source of acetylcholine (ACh) which acts to the kidney, parasympathetic innervations and non-neuronal expression of choline acetyl transferase (ChAT) were examined in the rat kidney by using a neuronal tracing method and histochemical methods, respectively. Retrograde neuronal tracer Fluoro-gold was treated to the renal nerve ends by a micro-capsule, resulting that there were no positive cells in any parasympathetic nuclei in the medulla oblongata and spinal cords. On the other hand, ACh-synthesizing enzyme ChAT was localized to the renal cortical collecting ducts with uneven distributions in the tubules. These results suggest that the non-neuronal ACh may be predominant over the neuronal ACh in the rat kidney.