Recruitment of Polycomb-group complex for stable repressions

为了更好地了解PRC1的招募机制，我们重点研究了由PRC1组成的亚核结构域。已知H3K27me3是影响PRC1招募的因素之一。然而，Ezh2的条件性缺失实验并不支持H3K27me3是PRC1的关键招聘基因。另一方面，FRAP分析表明，约20%的Prc1组分在染色质上是不活动的，这意味着这种不活动的部分成为细胞分裂后的Prc1的记忆标签。因此，我们一直在研究这一假设

To better understand recruitment mechanisms of PRC1, we focused on subnuclear domains consisting of PRC1. To data H3K27me3 is known to be one of factors for PRC1 recruitment. Conditional deletion experiments of Ezh2 however did not support that H3K27me3 was a critical recruiter of PRC1. On the other hand, FRAP analysis showed that about 20% of PRC1 component was immobile on chromatin, implying that such an immobile fraction becomes a memory tag of PRC1 beyond cell division. We thus have been addressing this hypothesis