Investigation of the regulatory mechanisms of cellular energy metabolism by snail

蜗牛表达减少氧化磷酸化，增加MDCK细胞的葡萄糖依赖性。MDCK/Snail细胞显示控制丙酮酸进入TCA循环的丙酮酸脱氢酶（PDH）活性降低，抑制PDH的丙酮酸脱氢酶激酶1 （PDK1）启动子活性和表达增加。此外，MDCK/蜗牛细胞中TCA循环酶活性降低。因此，MDCK/蜗牛细胞比MDCK/neo细胞表现出更低的氧消耗和ATP水平。MDCK/蜗牛细胞中谷氨酰胺水解酶和脂肪酸合成酶的表达也有所降低。

Snail expression decreases oxidative phosphorylation and increases glucose dependency in MDCK cells. MDCK/Snail cells exhibited reduced activity of pyruvate dehydrogenase (PDH), which controls pyruvate entry into the TCA cycle, and increased promoter activity and expression of pyruvate dehydrogenase kinase 1 (PDK1), which inhibits PDH. In addition, the activities of TCA cycle enzymes were decreased in MDCK/snail cells. Consequently, MDCK/snail cells exhibited lower O2 consumption and ATP levels than MDCK/neo cells. MDCK/snail cells also showed reduced expression of enzymes essential for glutaminolysis and fatty acid synthesis.