Development andapplication of the new clinical laboratory procedure using saliva at home medical care-For the prevention of senile pneumonia-

唾液中CRP和人β防御素（hBD）浓度可用于老年性肺炎的早期发现和预防。CRP被认为是肺炎和hBD的炎症标志物，分别被认为是口腔清洁水平的指标。我们发现在炎症性疾病患者的唾液和血清中测量的CRP有很好的相关性（r=0.88,P < 0.001）。此外，当唾液中转铁蛋白浓度被限制在小于1 mg/ml时，这种关联变得更强（r=0.96,P < 0.001）。此外，还研究了几种用于测定带正电荷hBD的唾液预处理方法。用Tween 20和稀释的盐酸将hBD从带负电荷的物质中释放出来，如muchin。该预处理方法可准确测定唾液中hBD的浓度。提示唾液检测对老年肺炎的早期发现和预防具有重要意义。

Both CRP and human beta defensin(hBD) concentration in saliva were used for the early detection and prevention of senile pneumonia. CRP was recognized as an inflammatory marker of pneumonia and hBD, as an index of thecleanliness level in oral cavity, respectively. We found a good association between CRP measured in saliva and serum of an inflammatory disorder patients (r=0.88,P < 0.001). Furthermore, when the transferrin concentration in saliva was limited to a less than 1 mg/ml sample, the association was become stronger (r=0.96,P < 0.001). Moreover, some pretreating methods of saliva were examined for the measurement of hBD with positive charge. Tween 20 and diluted hydrochloric acid were used to release hBD from negative charged matter, such as muchin. Measurement of the exact hBD concentration in salivawas attained by this pretreating method. These results suggested that saliva test would be useful for the early detection and prevention of senile pneumonia.