Simultaneous sensitive detection of chelated medicines and poisonsby electrospray ionization tandem mass spectrometry without column separation

为了方便高亲水性药物和毒物的检测，我们开发了一种方法，在电喷雾电离质谱（ESI-MS）检测之前，将分析物生成螯合物，然后用极性有机溶剂提取它们。在研究过程中，我们注意到该方法可以同时检测几种分析物。在同时检测中，通常进行柱分离以分离分析物并去除杂质。串联质谱法（MS -MS）在一瞬间对前体离子和产物离子进行两次质谱检测，从而消除了两次杂质。大多数具有不同分子结构的分析物，虽然在第一次质谱分析中显示出相同的m/z，但在第二次质谱分析中显示出不同的产物离子。因此，我们的ESI-MS-MS同时检测方法不需要大约30分钟的柱分离。使用这种方法，三组分析物，即1。叠氮化氰离子基，2。铸币金属组，3。与甲状腺功能减退有关的离子群，在这三年中可以同时检测到。使用单一样品进行多分析物检测，无需柱分离，大大节省了法医和临床毒理学的时间和资源。

To facilitate the detection of highly hydrophilic medicines and poisons, we developed a method that produces chelate complexes of analytes and then extracts them with polar organic solvents before electrospray ionization mass spectrometric (ESI-MS) detection. During the study we noticed the possibility to detect several analytes simultaneously by the method. In the simultaneous detection, usually, column separation was performed to separate analytes as well as to eliminate impurities. In tandem mass spectrometry (MS -MS), MS detection was performed twice in a blink for precursor ion and product ion, and hence impurities were eliminated twice. Most analytes having different molecular structures showed different product ions at the 2ndMS even though they showed the same m/z at the 1stMS. Therefore, our simultaneous detection method by ESI-MS-MS did not requirecolumn separation that took about 30 min. Using this method, three groups of analytes, that is, 1. cyanide-azide ion group, 2. coinage-metal group, 3. ion group that related to hypothyroidism, could be detected simultaneously during these three years. Multi analytes detection using a single sample without column separation contributes greatly to the saving of time and resources in forensic and clinical toxicology.