Regeneration of Damaged Peripheral Nerve using Skeletal Muscle-Derived Multipotent Stem Cell co-using with Absorbable Tube

为了开发治疗不可逆长神经缺失的新方法，我们应用骨骼肌源性多能干细胞片丸(Sk-MSCSPs)作为一种新的自体神经移植的替代来源。我们用该管去细胞导管(70%乙醇脱水的食道粘膜)。4周后，导管被GFP+细胞/组织填充，移植的GFP+供体细胞/组织明显向受体神经近端伸展。8周后，上述指标进一步增强。这些结果有力地表明，Sk-MSCSP可能是最适合用于长时间神经间隙治疗的干细胞。因此，我们希望继续深入研究，以达到临床应用的目的。

To develop the new therapy for the irreversible long nerve deficit, we applied skeletal muscle-derived multipotent stem cell sheet pellets (Sk-MSCSPs), as a novel alternative source of nerve autograft. We used the tubeto the dcellularized conduit (dehydrated esophageal submucous membrane by 70% ethanol). After 4 weeks, conduit was filed by GFP+ cells/tissues, and engrafted GFP+ donor cells/tissues obviously stretch back to proximal portion of recipient nerve. After 8 weeks, theabove properties were more enhanced. These results strongly suggested that Sk-MSCSPs may be the best suitable stem cells for the long nerve gap therapy. Therefore, we want to continue further study with the aim of clinical application.