Functional analysis of Smoothened in osteosarcoma via signal cross talk

我们研究了Hedgehog通路在骨肉瘤细胞增殖、转移和肿瘤干细胞中的作用。我们在人骨肉瘤中发现了Hedgehog通路的激活。抑制Smoothened(SMO)通过调节细胞周期抑制骨肉瘤细胞的增殖。此外，SMO或GLI的药理抑制可阻止人横纹肌肉瘤细胞的增殖。此外，抑制GLI2可以阻止骨肉瘤细胞的增殖、侵袭和肺转移。我们已经发现其他信号相关分子通过Hedgehog信号激活来调节骨肉瘤细胞的转移。

We have examined the function of Hedgehog pathway in osteosarcoma cell proliferation, metastasis, and cancer stem cells. We found activation of Hedgehog pathway in human osteosarcoma. Inhibition of Smoothened (SMO) prevented osteosarcoma cell proliferation by cell cycle regulation in vitro and in vivo. In addition, pharmacological inhibition of SMO or GLI prevents human rhabdomyosarcoma cell proliferation. Furthermore, inhibition of GLI2 prevents osteosarcoma cell proliferation, invasion, and lung metastasis. We have already found that other signal related molecule regulate the osteosarcoma cell metastasis by Hedgehog signal activation.