New therapeutic strategy of pediatric surgical treatment for repair of tissue deficits and revascularization using autologous tissue grafting

应用光刻技术，以脐血生长内皮祖细胞和脐带Wharton凝胶间充质干细胞为材料，建立了具有毛细血管网络的羊膜片。与对照组相比，将具有毛细血管网络的羊膜片植入小鼠缺血后肢可增加再灌注率。从自体细胞体外工程构建的毛细血管网络可能在儿科外科的治疗中具有治疗潜力。

We established amnion sheets with capillary network by use of lithographic technique using outgrowth endothelial progenitor cells from umbilical cord blood and mesenchymal stem cells from Wharton’s jelly in umbilical cord. Implantation of the amnion sheet with capillary network into the ischemic hind limbs of mice increased reperfusion compared with controls. Implantation of a capillary network engineered ex vivo from autologous cells may have therapeutic potential for the treatment of pediatric surgery.