The pathogenesis induced by highly pathogenic avian influenza virus H5N1 hemagglutinin

近年来，高致病性禽流感病毒H5 N1引起了全世界对流感大流行的严重关注;然而，H5 N1致病机制在很大程度上是未知的。A/ Crow/京都/ 53/ 2004（H5 N1）[ Cr/ Ky（H5 N1）]对小鼠表现出高死亡率和体重减轻，而A/ Duck/ Hong Kong/ 820/ 80（H5 N3）则没有。此外，重组H5 N3病毒（rH 5 N3 H5 N1 HA）也显示了与Cr/ Ky（H5 N1）相似的结果。此外，Cr/ Ky（H5 N1）和rH 5 N3 H5 N1 HA也表明有效的病毒复制和肺出血，包括炎性细胞浸润。rH 5 N3 H5 N1 HA感染小鼠的促炎细胞因子水平高于Dk/ Hk（H5 N3）感染小鼠。总之，这些数据表明，H5 N1 HA扩大了小鼠中的病毒发病机制，并且该发病机制是基于小鼠中的病毒复制和促炎细胞因子平衡。

In recent years, the highly pathogenic avian influenza virus H5N1 has raised serious worldwide concern about an influenza pandemic ; however, the mechanism of H5N1 pathogenesis is largely unknown. A/ Crow/ Kyoto/ 53/ 2004(H5N1)[ Cr/ Ky(H5N1)] showed high mortality and weight loss toward mice, while A/ Duck/ Hong Kong/ 820/ 80(H5N3) did not. In addition, recombinant H5N3 viruses carrying the H5N1 HA gene(rH5N3H5N1HA) showed the similar result with that of Cr/ Ky(H5N1). In addition, Cr/ Ky(H5N1) and rH5N3H5N1HA also indicated efficient viral replication and lung hemorrhage including inflammatory cell infiltration. The levels of proinflammatory cytokine in mice infected with rH5N3H5N1HA was higher than mice infected with Dk/ Hk(H5N3). Taken together, these data suggest that H5N1HA broaden the viral pathogenesis in mice and this pathogenesis is based on viral replication and proinflammatory cytokine balance in mice.