Development of high sensitive MRI contrast agent encapsulating CEST agent into apoferritin cavity

设计并合成了具有不同电荷的稀土配合物作为新型化学交换和饱和转移剂。将其包封于脱铁铁蛋白空腔中，并在体外评价其作为CEST型MRI造影剂的性能。此外，用亲水性聚合物修饰脱铁铁蛋白的表面以延长其血液循环行为，并且我成功地利用增强的渗透和保留（EPR）效应将葡聚糖涂覆的脱铁铁蛋白递送到移植的肿瘤区域。使用肿瘤移植小鼠检查包封CEST试剂的脱铁铁蛋白MRI造影剂在体内的性能。

Lanthanoid complexes having various electric charges were newly designed and synthesized as new chemical exchange and saturation transfer(CEST) agents. The complexes were encapsulated into apoferritin cavity, and thier character as CEST type MRI contrast agent was evaluated in vitro. Further, surface of apoferritin is modified with hydrophilic polymers to extend its blood circulation behavior, and I succeeded to deliver the dextran-coated apoferritin to transplanted tumor region utilizing enhanced permeation and retention(EPR) effect. Performance of the apoferritin MRI contrast agent encapsulating CEST agent in vivo was examined using tumor-transplanted mouse.