The establishment of induction method from iPs cells to hepatocyte:For cell transplantation

近年来，原位肝移植是治疗终末期肝病最有效的方法。但是，该疗法存在供体短缺和免疫排斥的问题。因此，肝细胞移植被列为肝移植的替代疗法，我们研究了肝细胞移植。首先，我们选择iPS细胞和间充质干细胞作为细胞来源，尝试并成功地在体外诱导人诱导多能干细胞和人间充质干细胞分化为肝细胞样细胞。特别是，提供了细胞外基质和细胞因子的细胞，RT-PCR和免疫组化检测白蛋白和其他肝细胞标志物的表达。我们将这些肝细胞样细胞移植到SCID大鼠的肝脏中。在移植了来源于间充质干细胞的肝细胞样细胞的大鼠中，我们在肝脏中检测到白蛋白阳性细胞，在这些大鼠中的一些大鼠中，人白蛋白的血清水平升高。然后，在移植了来源于iPS细胞的肝细胞样细胞的大鼠中，结果表明，iPS细胞和间充质干细胞可作为肝细胞的细胞来源移植

Recently, orthotopic liver transplantation is unique and the most efficient treatment for end-stage liver disease. But, the therapy has the issues of donor shortage and immune rejection. Then, a hepatocyte transplantation is listed as a alternative therapy for liver transplantation, we investigated hepatocyte transplantation.First, we selected iPS cells and mesenchymal stem cells as a cell source, and we tried and succeeded to induce differentiation of human induced pluripotent stem cells and human mesenchymal stem cells to hepatocyte like cells in vitro.In particular, the cells which was provided with extra cellular matrix and cytokines, expressed albumin and other hepatocyte marker in RT -PCR and immunohistochemistry. We transplanted these hepatocyte like cells into livers of SCID rats. In rats which were transplanted hepatocyte like cells derived from mesenchymal stem cells, we detected the albumin positive cells in the liver, in some of those rats, the serum level of human albumin increased.And then, in rats which were transplanted hepatocyte like cells derived from iPS cells, the albumin positive cells were detected in the liver .This result indicated that we can use iPS cells and mesenchymal stem cells as a cell source of hepatocyte transplantation.