Molecular network analysis of salivary duct carcinoma for the control of distant metastasis

从microRNA阵列（四对唾液管癌和正常唾液腺区域）的结果来看，被报道为肿瘤miRNAs的miR-21和miR-23-24-27簇的表达增加，而被报道为肿瘤抑制miRNAs的miR-375、miR-1和miR-200家族的表达减少。为了分析miR-200家族调控的基因，我们使用miRNA靶点搜索程序和通路分析进行了计算机分析。结果提示miR-200家族调控的基因参与了细胞粘附。此外，通过对临床样本的微阵列分析整理出局灶黏附相关基因，细胞黏附中过表达的基因可能参与了涎腺导管癌的远端转移。这些结果提示，细胞粘附中过表达的基因可能是改善不良预后的新疗法的潜在靶点。

From the results of microRNA array(four pairs of the salivary duct carcinoma and normal salivary gland region), expressions of miR-21 and miR-23-24-27 cluster which have been reported as oncomiRs were increased, whereas expressions of miR-375, miR-1, and miR-200 family which have been reported as tumor suppressive miRNAs were decreased. In order to analyze the gene(s) regulated by miR-200 family, we performed in silico analysis with miRNA target search program and pathway analysis. The results suggested that genes regulated by miR-200 family are involved in cell adhesion. Furthermore, collated focal adhesion-related genes with the microarray analysis of clinical samples, over-expressed genes in cell adhesion may be involved in distant metastasis that is the clinical features of salivary duct carcinoma. These results suggested that the over-expressed genes in cell adhesion might be potential targets of novel treatment for improving the poor prognosis.