Quantitative analysis and the investigation of the pneumococcal nasopharyngeal colonization in children.

链球菌肺炎(S。儿童鼻咽部定植的肺炎菌（Pneumoniae）是小儿急性中耳炎和鼻窦炎的重要致病菌。本研究采用实时荧光定量PCR方法对鼻咽部肺炎链球菌进行定量分析。在健康儿童中，肺炎链球菌浓度与年龄呈反比关系。另一方面，上呼吸道感染儿童的肺炎链球菌浓度高于健康儿童。此外，我们揭示了一种新的用于检测中耳液和鼻咽分泌物中肺炎球菌的快速抗原试验可以成为检测肺炎链球菌的有效工具。

Streptococcus pneumonia(S. pneumoniae) colonized in nasopharynx of children is an important pathogenic bacteria in pediatric acute otitis media and rhinosinusitis. In this study, we performed quantitative analysis of nasopharyngeal S. pneumonia by using real-time PCR method. In the healthy children, the concentration of S. pneumoniae showed an inverse relation with the age. On the other hands, the concentration of S. pneumoniae in the children with upper respiratory infection was higher than the healthy children. Moreover, we revealed that a new rapid antigen test for detection of pneumococci in middle ear fluids and nasopharyngeal secretions can be an available tool for detection S. pneumoniae.