Intraocular Concentrations of Cytokines and Chemokines in Rhegmatogenous Retinal Detachment and the Effect of Intravitreal Triamcinolone Acetonide

为探讨玻璃体内注射曲安奈德(IVTA)治疗孔源性视网膜脱离(RRD)前后房水中趋化因子水平的变化，对19例19眼RRD患者行IVTA治疗。分别于术前(基线)和术后1d检测房水中白细胞介素6(IL-6)、白细胞介素8(IL-8)、嗜酸性粒细胞趋化因子(Eosorin)、粒细胞集落刺激因子(G-CSF)、巨噬细胞趋化蛋白-1(MCP-1)、单核细胞趋化蛋白-1(MCP-1)、巨噬细胞炎性蛋白-1α(MIP-1a)、β(MIP-1b)、RANTES、肿瘤坏死因子-α(TNF-α)和血管内皮生长因子(VEGF)水平。房水中可检测到MIP-1b和血管内皮生长因子，且RRD患者房水中MIP-1b和VEGF的表达明显高于对照组。IVTA后第1天，IP-10、MCP-1和MIP-1b水平显著降低，且IP-10、MCP-1和MIP-1b水平下降呈正相关。IVTA能抑制RRD眼的IP-10、MCP-1和MIP-1b水平。IP-10、MCP-1和MIP-1b在房水中的变化相似，这些趋化因子可能是RRD治疗的额外靶分子。

To determine the aqueous humor levels of chemokines before and after an intravitreal injection of triamcinolone acetonide (IVTA)in eyes with rhegmatogenous retinal detachment (RRD).Nineteen eyes of 19 consecutive patients with RRD who underwent IVTA were studied. 17 eyes without retinal vascular disease served as control.The aqueous humor levels of interleukin-6 (IL-6), IL-8, eotaxin, G-CSF, GM-CSF, IFN-gamma, IP-10, monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1 alpha (MIP-1a), beta (MIP-1b), RANTES, TNF-alpha and vascular endothelial growth factor (VEGF)were determined before the IVTA (baseline)and at one day after the IVTA.At the baseline, IL-6, IL-8, IP-10, MCP-1, MIP-1b and VEGF were detected in the aqueous and were significantly higher in eyes with a RRD than in controls. One day after IVTA, the levels of IP-10, MCP-1 and MIP-1b were significantly reduced and the decreases in IP-10, MCP-1 and MIP-1b were correlated. IVTA was able to suppress the levels of IP-10, MCP-1 and MIP-1b in eyes with RRD. The alterations in the aqueous level of IP-10, MCP-1 and MIP-1b were similar and these chemokines might potentially be additional target molecules in therapy for RRD.