Development of vascularized artificial fat flap using adipose-derived stem cells

研究了脂肪来源的干细胞(ASCs)在人工真皮、含有游离脂肪的动静脉血管束和放置在组织工程室(TEC)中的带血管脂肪瓣上的作用。实验1用游离脂肪和动静脉血管束，实验2用血管脂肪，分为对照组和ASCs组。用1，1‘-二十八烷基-3，3，3’，3‘-四甲基吲哚甲醛高氯酸盐(DII)示踪血管干细胞。48和2.20±0。实验1中对照组和ASCs组分别为094 cm~3和1.30±0。19和1.36±0。22 cm^3，两个试验均无显著差异(p&gt；0。05)。组织病理学检查结果亦无明显差异。此外，在TEC再生软组织中未见DiI标记细胞。这些发现表明，ASCs在TEC早期就发生了坏死。

The effects of adipose-derived stem cells(ASCs) seeded on artificial dermis, arteriovenous vascular bundles with free fat, and a vascularized fat flap placed in a tissue engineering chamber(TEC) were investigated. The samples were divided into control and ASCs groups in Experiment 1 with free fat and arteriovenous vascular bundles, and Experiment 2 with vascularized fat. The amount of regenerated tissue was evaluated, and ASCs were traced using 1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine perchlorate(DiI).Results : The regenerated vascularized soft tissue volumes were 2. 12±0. 48 and 2. 20±0. 094 cm^3 in the control and ASCs groups in Experiment 1, respectively, and 1. 30±0. 19 and 1. 36±0. 22 cm^3 in Experiment 2, respectively, showing no significant difference in either experiment(p> 0. 05). No significant difference was also noted on histopathological examination. Moreover, no DiI-labeled cell was observed in the regenerated soft tissue in TEC. These findings suggest that ASCs were necrotized early in TEC.