Identification and localization of new human pulpal stem cell markers for regenerative medicine studies

从原代培养的人牙髓细胞中获得50个克隆，培养很长一段时间，直到它们衰老。分析了具有代表性的2个具有不同分化能力的克隆间的基因表达谱。一个具有成骨、软骨和脂肪分化能力的克隆与另一个具有成骨、软骨和脂肪分化能力的克隆相比，显示出细胞周期和细胞分裂相关基因的高表达。新的鉴定人牙髓干细胞的候选基因可能包括在细胞周期和细胞分裂相关基因及其转录因子的范围内。

Fifty clones derived from human dental pulp cells in primary culture were incubated for a long period until they senesced. Gene expression profiles were analyzed between representative 2 clones which had different differentiation capacities each other. One clone exhibiting osteogenic, chondrogenic and adipogenic differentiation abilities displayed high expression of cell cycle- and cell division-relating genes compared with another clone with osteogenic, chondrogenic but not adipogenic differentiation abilities. New candidate genes for identifying human dental pulp stem cells may be included within the range of cell cycle- and cell division-relating genes and their transcriptional factors.