The new strategy of the replacement therapy for prevention of dental caries

为了构建替代治疗预防龋齿的多糖降解酶产生菌，我们首先鉴定并分析了一种突变酶（水不溶性葡聚糖降解酶）的编码基因。其次，利用质粒表达系统构建了多糖降解酶过表达菌。

In order to construction of polysaccharide degradation enzymes producing bacteria for caries prevention by replacement therapy, we firstly identified and analyzed the genes encoding a mutanase (water insoluble glucan degradation enzyme). Secondarily, we constructed the bacterium which is overexpress of polysaccharide degradation enzymes by using plasmid-borne expression system.