Establishment of a method to promote osteoblast differentiation using tryptophan derivatives and Dex, and elucidation of the mechanisms of them

在这项研究中，我们研究了新合成的色氨酸衍生物和地塞米松(地塞米松，一种合成的糖皮质激素)对成骨细胞分化的影响。矿化表明，色氨酸衍生物和地塞米松促进了成骨细胞的终末分化。但未观察到间充质干细胞(MSC)向成骨细胞分化的诱导作用。此外，转录因子Osterix在色氨酸衍生物和地塞米松诱导成骨细胞分化的机制中发挥了重要作用。此外，我们还发现这些药物显著诱导了成骨标志基因骨涎蛋白(BSP)和碱性磷酸酶(ALP)在MSCs与成骨细胞共培养中的表达水平。

In this study, we investigated the effect of newly synthesized tryptophan derivatives and dexamethasone (Dex), a synthetic glucocorticoid, on osteoblast differentiation. The tryptophan derivatives and Dex enhanced terminal osteoblast differentiation, as indicated by mineralization. However, no inductive effect was observed on commitment of mesenchymal stem cells (MSC) into osteoblast differentiation was observed. Furthermore, it was suggested that a transcription factor, Osterix, played an important role in the mechanism by which tryptophan derivatives and dexamethasone induced terminal osteoblast differentiation. In addition, we demonstrated that these agents significantly induced mRNA expression levels of the osteogenic marker genes bone sialoprotein (BSP) and Alkaline Phosphatase (ALP) in MSCs co-cultured with osteoblasts.