The identification of the target protein of N-α-acetyltransferase and the devel opment of a newly therapy by the control of N-α-acetylome

本研究发现5-FU可降低喉癌细胞株HEp-2中N-α-乙酰转移酶（NATH）的mRNA和蛋白水平。提示NATH对乙酰组的调控是肿瘤细胞调控的重要因素。本研究的目的是开发一种基于乙酰组调控的新疗法。为了鉴定NATH靶蛋白，将siNATH和阴性对照siRNA（siNC）转染HEp-2细胞，收集蛋白质，对蛋白质组学分析中表达下调的两个蛋白（HSP 70、HSP 90）和mRNA表达下调的TS进行Western blot分析。Western blot结果显示，siNATH组和siNC组的蛋白表达量无明显差异。

I found out that 5-FU the decreases mRNA and the protein revel of N-α-acetyltransferase(NATH) in laryngeal cancer origin HEp-2. It is suggested that the control of acetylome by NATH is an important factor for regulation of cancer cells. The aim of this study is to develop of a newly therapy based on control of acetylome.The research results are as follows. In order to identify NATH target protein, protein was collected from HEp-2 which were transfected with siNATH and the negative control siRNA(siNC) and Western blot was done in two protein(HSP70, HSP90) which was admitted downregulation in proteomic analysis and TS which was admitted the downregulation of mRNA expression. However, the difference of the amount of protein revel was not accepted in siNATH group and siNC group in Western blot.