Regulation of protein function in living cells by using a unique enzyme reaction

古菌硫化叶菌的生物素化反应具有独特的特点，即生物素连接酶与其生物素化底物蛋白形成稳定的复合物。在本工作中，通过使用这种独特的生物素化反应，我们试图构建能够在活细胞中调节靶蛋白功能的系统。作为研究的结果，我们成功地调节了活细胞中靶蛋白的定位。

Biotinylation reaction from archaeon Sulfolobus tokodaii has a unique characteristic that the biotin ligase forms a stable complex with its biotinylated substrate protein. In the present work, by using this unique biotinylation reaction, we attempted to construct the system that enables the regulation of the functions of the target proteins in living cells. As a result of the study , we have succeeded in the regulation of localization of a target protein in living cells.