Development of Microchip Electrophoresis.Mass Spectrometry Analysis of Biomolecules Using Nanoparticles

在本研究中，纳米颗粒分别应用于毛细管电泳（CE）和毛细管电色谱（CEC）分析对映体和磷酸肽。为了对映体进行CEC分析，采用乳化法制备了cinchonidine （CCND）纳米晶体，并将其固定在熔融二氧化硅毛细管和微芯片的内表面。利用CCND纳米晶固定相，成功地实现了外消旋氨基酸的CEC对映分离。另一方面，在磷酸肽的CE分析中，采用部分注入二氧化钛纳米粒子的在线预富集技术提高了检测灵敏度。通过在分离柱中注入短塞的二氧化钛分散体，可以很好地浓缩ss-酪蛋白释放的ATP和磷酸肽

In this study, nanoparticles were applied to capillary electrophoresis (CE) and capillary electrochromatography (CEC) analysis of enantiomers and phosphopeptides, respectively. For the CEC analysis of enantiomers, cinchonidine (CCND) nanocrystals were prepared by an emusion method to immobilize onto the inner surface of fused silica capillaries and microchips. By using the CCND nanocrystals stationary phase, the CEC enantioseparations of racemic amino acids were successfully achieved. In the CE analysis of phosphopeptides, on the other hand, an on-line preconcentration technique using partially injected titania nanoparticles was developed to improve the detection sensitivity. By injecting a short plug of the titania dispersion into the separation column, ATP and phosphopeptide released from ss-casein were well concentrated