Control of Nucleation Rate for Protein Crystals under Application of an External AC Electric Field

蛋白质结晶的新技术的建立是必要的。在以前的工作中，我们已经成功地在外加交流电场的情况下控制了鸡蛋清溶菌酶晶体的成核速度。此外，我们还指出，界面处形成的双电层(EDL)对这种晶化技术起着关键作用。因此，在本课题中，我们通过使用不同的沉淀剂和浓度来调节界面上形成的EDL的厚度来控制外加电场对形核率的影响。此外，我们还利用该结晶技术控制了猪胰岛素的成核速率。这表明这种结晶技术也适用于几乎所有的蛋白质。

The establishment of novel techniques for the crystallization of proteins is necessary. In previous work, we have succeeded in controlling the nucleation rate of hen-egg white lysozyme crystals under application of an external ac electric field. Moreover, we have also suggested that the electric double layer (EDL)formed at the interface play a key role for this crystallization technique. In this subject, therefore, we controlled the effect of the external electric field on the nucleation rate by regulating the thickness of the EDL formed at the interface by using the various precipitants and concentrations. In addition, we also controlled the nucleation rate for porcine insulin using this crystallization technique. This suggests that this crystallization technique be also adapted to almost all proteins.